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mouse granzyme b specific kit  (RayBiotech inc)


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    Structured Review

    RayBiotech inc mouse granzyme b specific kit
    ELISA-based <t>granzyme</t> quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).
    Mouse Granzyme B Specific Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+granzyme+b+specific+kit/bio_rxiv__2023__10__14__560755-271-31-36?v=RayBiotech+inc
    Average 90 stars, based on 1 article reviews
    mouse granzyme b specific kit - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes"

    Article Title: IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes

    Journal: bioRxiv

    doi: 10.1101/2023.10.14.560755

    ELISA-based granzyme quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).
    Figure Legend Snippet: ELISA-based granzyme quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture



    Similar Products

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    RayBiotech inc mouse granzyme b specific kit
    ELISA-based <t>granzyme</t> quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).
    Mouse Granzyme B Specific Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+granzyme+b+specific+kit/bio_rxiv__2023__10__14__560755-271-31-36?v=RayBiotech+inc
    Average 90 stars, based on 1 article reviews
    mouse granzyme b specific kit - by Bioz Stars, 2026-07
    90/100 stars
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    ELISA-based granzyme quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).

    Journal: bioRxiv

    Article Title: IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes

    doi: 10.1101/2023.10.14.560755

    Figure Lengend Snippet: ELISA-based granzyme quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).

    Article Snippet: Conditioned media from co-culture of OT-I activated CD8+ T cells with mouse B cell lymphoma (OVAp pulsed and non-pulsed controls) were assessed for the concentrations of Granzyme B by ELISA using mouse Granzyme B specific kit (RayBiotech, Cat# ELM-GranzymeB-1), according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture